Conditional down-regulation of terpene nucleoside biosynthesis genes.

<p>(A) Meta-analysis of published transcriptomics data showed normalized expression of <i>Rv3376</i>, Rv3377c, or <i>Rv3378c</i> in glucose medium during log-phase (blue with blue dotted line to demarcate the reference condition) versus conditions found to repress transcription >4-fold among the 231 unique conditions [<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3002813#pbio.3002813.ref039" target="_blank">39</a>,<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3002813#pbio.3002813.ref040" target="_blank">40</a>]. (B) Heatmap of the most significantly changed genes in a contrast of Δ<i>Rv3378c</i> to Mtb H37Rv, with genes clustered by hierarchical clustering after transcriptiomics using RNAseq. (C) Expression of the terpene nucleoside locus genes using quantitative reverse transcription PCR of mRNA in Mtb H37Rv and in the Δ<i>Rv3378c</i> or complemented Δ<i>Rv3378c</i>::<i>Rv3378c</i> strains, normalized to 16S ribosomal RNA. All pairwise contrasts were tested but only significant <i>p</i> values are shown (<i>t</i> test with Bonferroni correction). The data for S8 Fig can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3002813#pbio.3002813.s013" target="_blank">S1 Data</a>; BioProject accession PRJNA1146031 contains raw RNAseq data.</p> <p>(PDF)</p>