Exploring the regulating hyperlipidemia mechanism of functional components in Guizhou Miaojia rice wine based on GC-MS combined with network pharmacology

In this study, firstly, the main volatile active ingredients were selected from Guizhou Miaojia rice wine by gas chromatography-mass spectrometry (GC-MS) combined with SwissADME, the effect targets were predicted by SwissTargetPrediction, the hyperlipidemia disease targets were collected by combining OMIM, Drugbank, DisGeNET and other databases, and the intersection targets of the active component effect targets and the hyperlipidemia disease targets were obtained. Then, protein-protein interaction (PPI) network analysis was constructed by STRING and Cytoscape, and the Gene ontology (GO) and Kyoto Encyclopedia of Gene and Gene (KEGG) pathway enrichment analysis was performed using the DAVID platform. Finally, the network map of "Miaojia rice wine-component-target-pathway-disease" was constructed by combining KEGG pathway enrichment analysis, to explore the pharmacodynamic substance basis and potential action mechanism of Miaojia rice wine in regulating hyperlipidemia. The results showed that Guizhou Miaojia rice wine might play a role in regulating hyperlipidemia by using 3,4-dihydroxyphenyl diol, ethyl acid, ethyl hexanoate, trans-2-dodecenal as key active components to act on key targets such as peroxisome proliferator-activated receptor gamma (PPARG), interleukin-6 (IL-6) and 3-hydroxy-3-methyl glutaryl coenzyme A reductase (HMGCR) of pathways such as the peroxisome proliferator-activated receptor signaling pathway, cancer pathway, and adipocytokine signaling pathway.