Medullins A‑H,\nSesquiterpenes from <i>Perenniporia medulla-panis</i>, and\nTheir Cellular Signaling\nMechanism in HDF Cells

A chemical investigation of an ethyl acetate-soluble\nlayer in the\nculture broth of <i>Perenniporia medulla-panis</i> resulted\nin the isolation of eight novel sesquiterpenes conjugated Gly (<b>1</b>), l-Val (<b>2</b>), l-Ala (<b>3</b>), l-Tyr (<b>4</b>), l-Thr (<b>5</b>), l-Ile (<b>6</b>), l-Leu (<b>7</b>), and l-Phe (<b>8</b>). Elucidation of their\nstructures was performed through comprehensive spectroscopic analysis.\nThe absolute configuration of the sesquiterpene skeleton was ascertained\nusing modified Mosher’s methods. The configurations of the\namino acid units in compounds <b>2</b>–<b>8</b> were identified through acid hydrolysis followed by LC-MS analysis\nemploying Marfey’s method. Compounds <b>1</b>–<b>3</b> and <b>5</b>–<b>8</b> showed significant\nregulating effect on MAP kinase activity (p-ERK and p-JNK) in human\ndiploid fibroblast (HDF) cells.