Weight Differences-BasedMulti-level Signal Profilingfor Homogeneous and Ultrasensitive Intelligent Bioassays

Current high-sensitivity immunoassay protocols often involve complex signal generation designs or rely on sophisticated signal-loading and readout devices, making it challenging to strike a balance between sensitivity and ease of use. In this study, we propose a homogeneous-based intelligent analysis strategy called Mata, which uses weight analysis to quantify basic immune signals through signal subunits. We perform nanomagnetic labeling of target capture events on micrometer-scale polystyrene subunits, enabling magnetically regulated kinetic signal expression. Signal subunits are classified through the multi-level signal classifier in synergy with the developed signal weight analysis and deep learning recognition models. Subsequently, the basic immune signals are quantified to achieve ultra-high sensitivity. Mata achieves a detection of 0.61 pg/mL in 20 min for interleukin-6 detection, demonstrating sensitivity comparable to conventional digital immunoassays and over 22-fold that of chemiluminescence immunoassay and reducing detection time by more than 70%. The entire process relies on a homogeneous reaction and can be performed using standard bright-field optical imaging. This intelligent analysis strategy balances high sensitivity and convenient operation and has few hardware requirements, presenting a promising high-sensitivity analysis solution with wide accessibility.