Dual Gold Nanostructures-Based\nStretchable Electrochemiluminescence\nSensors for Hydrogen Peroxide Monitoring in Endothelial Mechanotransduction

Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) release\nduring blood\nflow is commonly provoked by the cyclic stretch and dynamic shear\nstress of endothelial cells and is of vital significance for maintaining\nvascular function. Flexible and stretchable electrochemical sensors\nshow great capability in retrieving mechanical stimulation-induced\nH<sub>2</sub>O<sub>2</sub> variation; however, cell secretions, especially\nelectroactive constituents’ interferences, remain a big concern\nfor sensing accuracy. Herein, we developed a stretchable electrochemiluminescence\n(ECL) sensor by synthesizing L012-reduced gold nanospheres and decorating\nthem onto a polydimethylsiloxane film-supported gold nanotubes substrate\n(Au NTs/PDMS) to form dual gold nanostructure-modified meshwork interface.\nGiven the specific reaction between L012 and H<sub>2</sub>O<sub>2</sub>, the as-prepared Au-L012/Au NTs/PDMS exhibited outstanding selectivity\ntoward H<sub>2</sub>O<sub>2</sub> quantification. Through culturing\nhuman umbilical vein endothelial cells (HUVECs), real-time monitoring\nof transient H<sub>2</sub>O<sub>2</sub> release from mechanically\nsensitive HUVECs in stretching states was realized. This work successfully\nincorporated the ECL sensing model into in situ cellular sensing,\ntherefore expanding the application mode of the ECL approach for health\ncare and biomedical investigation.