Multiomics Combined\nwith Expression Pattern Analysis\nReveals the Regulatory Response of Key Genes in Potato Jasmonic Acid\nSignaling Pathways to Cadmium Stress

Jasmonic acid (JA) is an endogenous phytohormone that\nregulates\nplant physiological metabolism and stress response processes, either\nindependently or through hormone crosstalk. Our phytohormone assay\nand transcriptome–metabolome analysis revealed the key genes\nand metabolites involved in the JA pathway in response to 0–250\nμM cadmium (Cd) in potato seedlings. Transcriptome gene set\nenrichment and gene ontology analysis indicated that JA-related genes\nwere significantly enriched. Specifically, members from the <i>StOPR</i> and <i>StJAZ</i> gene families showed pronounced\nresponses to Cd stress and methyl jasmonate treatment. As a negative\nregulatory transcription factor of the JA signaling pathway, <i>StJAZ14</i> exhibited a decreasing trend under Cd stress. Yeast\ntwo-hybrid assay identified an interaction between StJAZ14 and StBZR1,\nwhich is located on the brassinolide pathway. In addition to unveiling\nthe critical role of the JA pathway in regulating potato response\nto Cd stress, the functional mechanism was preliminarily explored.