Embracing Nature’s\nWisdom: Liposome-Mediated\nAmplification of Electrochemiluminescence for the Sensitive and Selective\nImmunoassay of Serum Amyloid A

Serum amyloid A (SAA) is a key biomarker for diagnosing\ninflammatory\nresponses in diseases like influenza and COVID-19. An electrochemiluminescence\n(ECL) biosensor has been constructed for signal enhancement in SAA\ndetection by encapsulating 4,4′,4″,4‴-(1,3,6,8-pyrenetetrayl)\ntetrakis-benzoic acid (TBAPy) into liposomes. Such biomimetic encapsulation\nshields the biologically important membrane to avoid aggregation of\nTBAPy and prevents quenching. It would enhance the ECL signal’s\nstability and intensity, resulting in a 5-fold signal increase with\na relative standard deviation (RSD) of 1.71%. The biosensor has shown\nvery good specificity and sensitivity toward SAA detection. The estimated\ndetection limit is 0.188 pg/mL. This innovative approach integrates\nbiomimicry with ECL technology and represents a leap in developing\nthe next generation of diagnostic tools with superior sensitivity\nand specificity.