Antisenescence Expansion\nof Mesenchymal Stem Cells\nUsing Piezoelectric β‑Poly(vinylidene fluoride) Film-Based\nCulture

Regenerative therapies based on mesenchymal stem cells\n(MSCs) show\npromise in treating a wide range of disorders. However, the replicative\nsenescence of MSCs during <i>in vitro</i> expansion poses\na challenge to obtaining a substantial quantity of high-quality MSCs.\nIn this investigation, a piezoelectric β-poly(vinylidene fluoride)\nfilm-based culture plate (β-CP) was developed with an antisenescence\neffect on cultured human umbilical cord-derived MSCs. Compared to\ntraditional tissue culture plates (TCPs) and α-poly(vinylidene\nfluoride) film-based culture plates, the senescence markers of p21,\np53, interleukin-6 and insulin-like growth factor-binding protein-7,\nstemness markers of OCT4 and NANOG, and telomere length of MSCs cultured\non β<i>-</i>CPs were significantly improved. Additionally,\nMSCs at passage 18 cultured on β-CPs showed significantly better\nmultipotency and pro-angiogenic capacities in vitro, and higher wound\nhealing abilities in a mouse model. Mechanistically, β-CPs rejuvenated\nsenescent MSCs by improving mitochondrial functions and mitigating\noxidative and glycoxidative stresses. Overall, this study presents\nβ-CPs as a promising approach for efficient and straightforward\nantisenescence expansion of MSCs while preserving their stemness,\nthereby holding great potential for large-scale production of MSCs\nfor clinical application in cell therapies.