Exploring the\nInterfacial Dynamics of Unilamellar\nand Multilamellar Cationic Liposomes on SiO<sub>2</sub> and Their\nInteractions with Membrane-Active Peptide

Understanding the interplay between\nlipid assemblies and solid\nsupports is crucial for advancing model membrane systems and biomedical\napplications. This study investigates the interfacial behaviors of\nunilamellar and multilamellar cationic liposomes on silicon dioxide\nand their interactions with a membrane-active AH peptide. Using QCM-D\nmonitoring, unilamellar liposomes were found to rapidly form SLBs\nthrough one-step adsorption kinetics, whereas multilamellar liposomes\nexhibited slower adsorption. Further addition of liposomes caused\nfusogenic interactions with SLBs, where multilamellar liposomes formed\nmore rigid lipid membranes. Upon AH peptide exposure, unilamellar-based\nlipid membranes showed higher susceptibility to structural transformations,\nachieving complete SLB formation, while multilamellar-based lipid\nmembranes displayed reduced sensitivity and retained residual viscoelastic\ncomponents, indicative of incomplete SLB formation. These findings\nunderscore the significant influence of liposome lamellarity on their\ninterfacial dynamics and peptide interactions, crucial for designing\neffective lipid-based delivery and sensing systems.