Microfluidics-Based\nIonic Catch and Release Oligosaccharide\nSynthesis (ICROS-Microflow) to Expedite Glycosylation Chemistry

A continuous microfluidic glycosylation strategy that\nrequires\nno chromatography between steps and significantly expedites glycosylation\nchemistry is described. This practical approach incorporates the advantages\nof imidazolium-based chromatography-free purification and in situ\nmass spectrometry reaction monitoring to achieve fast reaction optimization\nand shorter reaction times. We demonstrate the utility of this strategy\nin the synthesis of a series of glycoside targets, including an α-(1,6)-trimannoside\nand a branched Man<sub>5</sub> oligomannoside, within 1 day.