Human Serum AlbuminLoaded with Fatty Acids RevealsComplex Protein–Ligand Thermodynamics and Boleadora-Type SolutionDynamics Leading to Gelation

Using an electron paramagnetic resonance (EPR) spectroscopic strategy that has been developed for core–shell polymers, the complexity of the binding of fatty acids to human serum albumin (HSA) is characterized in detail. We unravel the internal dynamics of HSA solutions with fatty acids by applying continuous wave EPR (CW EPR) from which we derive a consistent thermodynamic interpretation about fatty acid interactions with HSA in the investigated temperature range of 5–97 °C. Additionally, data from CW EPR are corroborated by dynamic light scattering (DLS), differential scanning calorimetry (DSC) and nanoscale distance measurements using double electron–electron resonance (DEER) spectroscopy. We discuss our data in light of decades of biophysical studies on albumin and aim at drawing a complete functional and dynamic picture of HSA “at work”. This picture suggests that HSA is built from modular, rotationally decoupled domains that resemble an entangled three-piece <i>boleadora</i> in solution.