nQuant Enables\nPrecise Quantitative N‑Glycomics

N-glycosylation is a highly heterogeneous post-translational\nmodification\nthat modulates protein function. Defects in N-glycosylation are directly\nlinked to various human diseases. Despite the importance of quantifying\nN-glycans with high precision, existing glycoinformatics tools are\nlimited. Here, we developed nQuant, a glycoinformatics tool that enables\nlabel-free and isotopic labeling quantification of N-glycomics data\nobtained via LC-MS/MS, ensuring a low false quantitation rate. Using\nthe label-free quantification module, we profiled the N-glycans released\nfrom purified glycoproteins and HEK293 cells as well as the dynamic\nchanges of N-glycosylation during mouse corpus callosum development.\nThrough the isotopic labeling quantification module, we revealed the\ndynamic changes of N-glycans in acute promyelocytic leukemia cells\nafter all-trans retinoic acid treatment. Taken together, we demonstrate\nthat nQuant enables fast and precise quantitative N-glycomics.