Automated Synthesis of Aβ Imaging Agent 18F-AV45 Based on NEPTIS Perform Module

To meet the clinical application requirements，the Aβ-Amloid imaging agent 18F-AV45 was synthesized automatically using NEPTIS perform synthesis module .In the pre-written program，the AV105 was used as precursor to obtain the 18F-intermediate through nucleophilic substitution reaction with 18F−，followed by hydrolysis with hydrochloric acid，neutralization with base to obtain the crude product of 18F-AV45，which was next purified by solid-phase extraction and semi-preparative high-performance liquid chromatography (HPLC). Finally，18F-AV45 injection solution was obtained through sterile filtration，followed by quality control analysis. The average production time of 18F-AV45 preparation was 60 min，the no-corrected efficiency was (32.1±2.1)% (n=5)，the radiochemical purity was over 98%，and the appearance color，pH value，in vitro stability，bacterial endotoxin，and residual solvents all met the requirements. 18F-AV45 could be synthesized automatically and simply by NEPTIS perform module with good repeatability and the product meets the requirements for clinical use.