Characterization and Antimicrobial Activity of Silverrods Nano: Synthesized in Green Ocimum sanctum Leaf Extract

Ocimum sanctum (OS) Linn, is a medicinal herb that has been used in traditional medicine science ancient time. It contains an array of complex and diverse biologically active phytochemical constituents that can act as a reducing and stabilizing agent for synthesizing metal nanoparticles (NPs). Herein, OS aqueous leaf extract was used to synthesize eco-friendly round and rod-shaped Ag NPs. The synthesized spherical and rod-shaped Ag NPs were analyzed by indirect methods by analysis of plasma band (UV-vis spectra) and thin films X-ray diffraction (XRD) peaks. Whereas, for direct methods, morphologies of synthesized Ag NPs were recoded using HR-TEM. Ag NPs demonstrated dose-dependent antibacterial efficacy against bacteria ( S. aureus). The MIC and MBC values of spherical Ag NPs against 106CFU/mL of S. aureus were found to be 2.74 and 3.80µ g/mL, respectively. However, MIC and MBC values of Ag nano-rod were observed at 7.20µ g/mL and 13µ g/mL, respectively. SEM micrographs indicate that morphologies of spherical shape and nano-rod Ag NPs treated S. aureus showed breakdown, merge, and elongated morphologies with the presence of Ag NPs (nano-rod) inside of the S. aureus.