One anti-tumour C21 steroidal glycoside from <i>Cynanchum wallichii</i> Wight

The roots of <i>Cynanchum wallichii</i> Wight is rich in C21 steroidal glycosides according to previous research. In this study, a systematic chemical study was performed on the roots of <i>C. wallichii</i>, leading to the isolation of one previously undescribed C21 steroidal glycoside compound (<b>1</b>) and a known compound, gagamine (<b>2</b>). <i>In vitro</i> bioassay indicated that compound <b>1</b> showed a strong inhibitory effect on AGS cells, with the IC₅₀ value 7.8 μM.