Additional file 3 of Identification of the methionine transporter MetQ in Streptococcus suis and its contribution to virulence and biofilm formation

Additional file 3. Confirmation of the metQ knock-out mutant. (A) Schematic representation of the metQ gene context in S. suis strain P1/7 and P1/7ΔmetQ mutant, indicating the position of the primers used for PCR validation. Agarose gels showing the PCR products amplified using primers (B) metQ_L-Fw and metQ_R-Rev and (C) metQ_Fw and metQ_Rev primers in both the wildtype and ΔmetQ mutant. Primer sequences and predicted amplicon sizes are specified in Additional file 1.