Additional file 13 of KDM6A facilitates Xist upregulation at the onset of X inactivation

Additional file 13 Supplemental figure S7. Characterization of KDM6A binding, expression and protein levels during differentiation of Tsix-stop cells. (A) Venn diagram comparing number of peaks of KDM6A in wt Tsix-stop cells at D4 of differentiation to H3K27me3 peaks in wt and Kdm6a KO cells at D15 of differentiation. (B) Same analysis as in (A) but using KDM6A binding at D7 for comparisons. (C) KDM6A enrichment profiles at Foxr2 and Igsf9, two genes known to be regulated by KDM6A. D4 signal and peaks in wt Tsix-stop cells are shown. Scales are shown in the upper right corner. (D) Top, protein blots of KDM6A in wt Tsix-stop (top) during differentiation. Adjusted density was calculated by normalizing levels to the GAPDH loading control using ImageJ (middle). Bottom, histograms of qRT-PCR of Kdm6a expression in two wt Tsix-stop replicates during differentiation. Expression was normalized using Actinβ and expression relative to D0 is shown.