Additional file 11 of Heterochrony in orthodenticle expression is associated with ommatidial size variation between Drosophila species

Additional file 11: Fig. S6. Effects of loss and gain of otd expression in ommatidial structure and facet size in D. melanogaster. Loss of otd causes defects in ommatidia structure. (a-c) Knockdown of otd by expressing UAS-miR-otd with GMR-GAL4 driver in D. melanogaster eye. (a-a’) GMR-GAL. (b-b’) GMR-GAL/ UAS-miR-otd shows a rough eye phenotype due to defects in ommatidia arrangements. (c-c’) GMR-GAL/ UAS-miR-otd phenotype is rescued by co-expressing UAS-otd. (d-d’) otd imutant mitotic clones also show defects in ommatidia size and organisation. (e) Comparison of facet area from eyes of UAS-otd (“+, otd”), ato3’FL-GAL4 (“ato>+) and UAS-otd; ato3’FL-GAL4 (“ato>otd”) female adults. Facet area is significantly larger in the experimental genotype (“ato>otd”) than in the two control genotypes. See main text for details.