Additional file 1 of Dorsal hyperintensity and iron deposition patterns in the substantia nigra of Parkinson’s disease, idiopathic REM sleep behavior disorder, and Parkinson-plus syndromes at 7T MRI: a prospective diagnostic study

Additional file 1. eMethods. Table S1 Demographic and clinical characteristics in the pooled dataset. Table S2 Inter-observer reliability of the seven DNH assessment methods in the pooled dataset. Table S3 Visual assessment of the DNH in the development cohort at echo 1-echo 4. Table S4 Visual assessment of the DNH in the pooled dataset at echo 1-echo 4. Table S5 Visual assessment of the DNH in early-stage PD subtypes in the pooled dataset at echo 2. Table S6 Predominant side of DNH impairment and motor symptoms. Table S7 Diagnostic and differential diagnostic performances of the seven DNH assessment methods in the development cohort. Table S8 Diagnostic and differential diagnostic performances of the seven DNH assessment methods in the pooled dataset. Table S9 Validation of diagnostic and differential diagnostic performances of the DNH abnormality. Table S10 Subgroup analyses of diagnostic and differential diagnostic performances of the seven DNH assessment methods in age- and education-matched participants aged over 60. Table S11 Follow-up evaluations of iRBD patients. Fig. S1 Flowchart of participant inclusion. Fig. S2 3D gradient-echo T2* images (echo 1-echo 4) for a representative case from each patient and healthy control groups. Fig. S3 ROC curves for the three DNH rating scales at echo 2 in the pooled dataset. Fig. S4 Receiver operating characteristic curves for the optimal DNH rating scale in the development cohort, its performance in the validation cohort, and its reassessment in the pooled dataset. Analysis S1 Comparison of participant characteristics. Analysis S2 Comparison of diagnostic and differential diagnostic performance between T2* echoes. Analysis S3 Correlation analysis between clinical characteristics and DNH scores in PD and MSA-C patients. Analysis S4 Comparison of clinical characteristics in MSA-P and PSP patients regarding the detectability of the DNH.