Additional file 4 of The tryptophan-aspartate (WD) repeat domain of bovine Coronin-1A promotes mycobacterial survival by inhibiting calcium signaling-mediated phagosome-lysosome fusion

Additional file 4. The coiled-coil domain mediates trimer formation of bCoronin-1A and inhibits phagolysosomal fusion. (A-B) SDS-PAGE and native-PAGE were used to detect the molecular forms of bCoronin-1A, ΔW and ΔC proteins. (C) Immunofluorescence localization of M.tb after 4 h of infection in cells overexpressing bCoronin-1A, ΔC and control cells. (D) Statistical analysis of the percentage of M.tb co-localized with lysosomes in the above cells. Data were analyzed by t-test and presented as the mean ± SEM of three independent experiments. * P < 0.05, *** P < 0.001.