Additional file 1 of Highly suitable LFBK cells for African swine fever virus replication and type I interferon-induced immune studies

Additional file 1: Determination of the ASFV infectivity in LFBK cells or PAMs. (A) The LFBK cells were infected with 10-fold serially diluted from 1 × 10−8 to 1 × 10−1 diluents of ASFV for 3 to 5 days post-infection (dpi). A 50% tissue culture infectious dose (TCID50) was determined based on whether or not a cytopathic effect occurred. The TCID50 was calculated using the Reed and Muench method. (B) PAM cells were non-infected or infected with ASFV (MOI of 0.1). Representative immunofluorescence images of the ASFV p72 protein (green) and DAPI (blue) staining in PAM cells (48 hpi). The experiments were carried out in triplicates, and the results represent the means ± SD. Magnification: 200×.