Additional file 5 of Cytotoxicity induced by Aeromonas schubertii is orchestrated by a unique set of type III secretion system effectors

Additional file 5. Phylogenetic analysis of core proteins in API1 and API2 injectisomes. To analyze the phylogenetic relationships of SctN and SctC proteins from Aeromonas API1 and API2, minimum evolution trees based on p-distance were constructed using MEGA11 software. Protein sequences were compared to their orthologs in different bacterial species. The results indicate that AscN and AscC of API1 map within the Ysc family, named after Yersinia spp., while SctN and SctC of API2 belong to the SsaEsc family, which is characteristic for the SPI2-encoded T3SS injectisome in Salmonella enterica serovar Typhimurium.