Additional file 10 of Lumpy skin disease virus suppresses the antiviral response of bovine peripheral blood mononuclear cells that support viral dissemination

Additional file 10. Heat treatment of LSDV reduces the expression of viral genes in inoculated PBMCs. (A-C) PBMCs were inoculated for 1 h with infectious LSDV WT-GFP at MOI=1 or with the same LSDV dose that was heat-treated LSDV before inoculation. Relative changes in the levels of transcripts encoding the reporter gene GFP and viral genes (LSDV035 and LSDV84) at the time points indicated were determined by RT-qPCR. One-way ANOVA with the Sidak multi-comparison test was used to measure the significance level. * p < 0.05, ** p < 0.01 and ***p < 0.001.