Additional file 4 of Circ-0000197 derived from porcine milk small extracellular vesicles promotes intestinal barrier function by sponging miR-429

Supplementary Material 4: Fig. S1A circ-0000197 content in PM-sEV after ultrasonic treatment. Fig. S1B Sanger sequencing further validated the head-to-tail splicing characteristic of circ-0000197 in both porcine mammary gland tissue and PM-sEV. Fig. S1C The amplified products were processed by Rnase. Fig. S1D TEER changes after overexpression of circ-0000197 (n = 3). Fig. S1E TEER changes after inhibition of circ-0000197 (n = 3). Fig. S1F TEER changes after overexpression of miR-429 (n = 3). Fig. S1G TEER changes after inhibition of miR-429 (n = 3).