Additional file 4 of A 3D human iPSC-derived multi-cell type neurosphere system to model cellular responses to chronic amyloidosis

Supplementary Material 4: Figure S4: Aβ aggregates form within 7 days of oligomeric Abtreatment in hiNS. A: hiNS cell culture medium was supplemented with three different doses of oAβ. Only the highestconcentration resulted in robust formation of aggregated Aβpositive area in hiNS. One-way ANOVA followed by Holm-Sidak’s post-hoc test C: Three-dimensional reconstruction of Aβ aggregates in hiNS. While oAβ treatment results in plaque-like aggregates inside the tissue, supplementing cell culture media with pre-aggregated fibrillar Aβresults in large Aβ sheets covering the hiNS tissue which do not mimic plaque-like structures