PCR-Based Detection of Antibiotic Resistance Genes in Gram-Positive Bacteria Isolated from Bacteriospermic Semen Samples

The study focuses on identifying antibiotic resistance genes (ARGs) in Gram-positive bacteria from semen samples with confirmed bacteriospermia. PCR was used to detect resistance genes, and antimicrobial susceptibility testing helped correlate genotypic findings with phenotypic resistance. The goal was to assess the implications of these bacteria on male infertility and potential transmission through assisted reproductive technologies (ARTs). The results showed a significant presence of ARGs, underlining the importance of routine screening and judicious antibiotic use in reproductive health.