Additional file 3 of Lumpy skin disease virus suppresses the antiviral response of bovine peripheral blood mononuclear cells that support viral dissemination

Additional file 3. GFP expression in MDBK cells and primary fibroblasts infected with GFP recombinant viruses indicates viral replication. (A-H) MDBK cells (A-D) and primary bovine fibroblasts (E-H) were inoculated with recombinant LSDV WT-GFP and LSDV Vac-GFP at MOI=3 for 1 h (T=0). Infected cells were collected and analysed by flow cytometry at the time points indicated. Histograms representing LSDV WT-GFP-infected MDBK cells (A, B) and fibroblasts cells (E, F) show GFP build-up in infected cells at the indicated time points. The percentage of GFP-expressing cells (C, MDBK cells and G, fibroblasts cells) and the mean fluorescence intensity (D, MDBK cells and H, fibroblasts cells) are presented. Values in graphs were expressed as mean± SEM representing three biological replicates (C- D and G- H).