Additional file 3 of Effects of M. tuberculosis and HIV-1 infection on in vitro blood-brain barrier function

Additional file 3: HIV-1 infection of CNS cells. (A, B) Astrocytes, (C, D) hCMEC/d3 (endothelial cells), (E, F) HBVP (pericytes) and (G, H) HMC3 (microglia) were left uninfected, infected with (B, D, F, H) HIV-1Bal or (A, C, E, G) VSV-G pseudotyped HIV-1 for 24 h before being incubated for 3 h with Mtb followed by extensive washing. Cells were then incubated for 48 h before staining and analysis by flow cytometry. The percentage of HIV + and Mtb + HIV + cells are displayed in filled and opened bars, respectively. The mean and SEM for 5 to 6 different donors/passages are shown