Additional file 1 of Microbially produced imidazole propionate impairs prostate cancer progression through PDZK1

Supplementary Material 1: Figure S1 The effect of IMP on different prostate cells. A Safety assay of IMP on normal prostate cancer cells RWPE-1 cells. B Histogram depicting cell colony counts following stimulation with IMP for PC3 and DU145 cells. C EdU staining plots of DU145 cells after IMP stimulation. Scale bar, 200 μm. D Histogram showing EdU detection results for PC3 and DU145 cells. E Western blot of PDZK1 in tumour and normal tissues. F Apoptosis assay of PC3 and DU145 after 48h of treatment with the corresponding concentrations of IMP. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; ns, not significant.