Additional file 18 of Cancer-associated fibroblasts promote drug resistance in ALK-driven lung adenocarcinoma cells by upregulating lipid biosynthesis

Supplementary Material 18 Fig. S14: (A) Log2 ratios depicting the alterations in saturation index induced by lorlatinib. The saturation index is calculated on the basis of the sum of species with the same (un)saturation level (n = 3). (B) Cell viability analysis following the combined treatment of H2228 tumor spheroids with fatostatin, lorlatinib, and fibroblast CM (n = 3). (C) Quantification of lipid peroxidation in H2228 spheroids using C11 BODIPY. Hydrogen peroxide (H2O2) served as a positive control. All data are presented as mean ± SD. #, p ≤ 0.05; ####, p ≤ 0.0001 compared to corresponding DMSO controls. ns, not significant; *, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001; ****, p ≤ 0.0001 in comparison to lorlatinib treatment alone.