Additional file 3 of Cytotoxicity induced by Aeromonas schubertii is orchestrated by a unique set of type III secretion system effectors

Additional file 3. List of PCR primers used for plasmid construction via Gibson assembly in this study. Uppercase letters represent the PCR primer regions corresponding to chromosomal DNA of A. schubertii ATCC 43700, while lowercase letters in H1-fw and H2-rv indicate sequences homologous to the linearized pAX2 vector. Italic underlined letters in H1-rv and H2-fw correspond to sequences inserted into the plasmid, including the GSSG linker followed by HiBit-tag, as required.