Additional file 1 of The ABC transporter A7 modulates neuroinflammation via NLRP3 inflammasome in Alzheimer’s disease mice

Supplementary Material 1: Table S1. Primer sequences for genotyping of the Abca7 / ABCA7 locus. Table S2. Comparison of DNA and protein sequences of murine, human and the in-frame inserted construct. Start codon for translation in the mouse gene is located in exon 2. Fig. S1. Agarose gel showing different PCR fragment sizes for each genotype. Fig. S2. Isolation of CD11b+ microglial cells from 100-day-old APPPS1 and APPPS1-hA7ko mice. Fig. S3. NLRP3 inflammasome changes induced by ABCA7 total knockout versus conditional Cx3cr1-knockout in 200-day-old APPPS1 mice. Fig. S4. Correlations between soluble Aβ42 levels (TBS fraction) and mRNA expression of NLRP3 inflammasome activators P2rx7 (A) and Cd36 (B). Fig. S5. LPS stimulation induces NLRP3 inflammasome expression and pro/anti-inflammatorycytokines release in APPPS1-hA7ko microglia