Additional file 1 of Engineering of l-threonine and l-proline biosensors by directed evolution of transcriptional regulator SerR and application for high-throughput screening

Supplementary Material 1: Fig. S1. Protein structure modeling of SerR. Fig. S2. The distance between the residue F104 and the effector l-serine. Fig. S3. Flow cytometry analysis of the random mutation library of Hom. Fig. S4. Flow cytometry analysis of the random mutation library of ProB. Table S1. Strains and plasmids used in this study. Table S2. Primers used in this study. Table S3. Characterization of Hom mutants. Table S4. Characterization of ProB mutants.