Additional file 3 of A 3D human iPSC-derived multi-cell type neurosphere system to model cellular responses to chronic amyloidosis

Supplementary Material 3: Figure S3: hiMG differentiation timeline shows a gradual increase in IBA1 expression. A: hiMG were plated on coverslips and fixed at different time points during differentiation. Immunofluorescence for IBA1is detectable at all time points. Co-staining with tomato lectin- 488 confirms that lectin can be used to label hiMG in vitro. B: Western blot for IBA1 confirms stable expression in hiMG up to at least day 21. C: Stimulation of hiMG with LPS for 24 h results in significant TNFα and IL1β release confirming their capacity to react to proinflammatory stimuli. D: Full length blots for GAPDH, IBA1, β-actin, Il1β and TNFα. E: All individual blots for Il1β and TNFα used for the quantification in panel C