Additional file 6 of An antibody-drug conjugate targeting soluble and membrane-bound TGFα is effective against pancreatic tumors

Supplementary Material 6: Fig. 6. Internalization of anti-TGFα-MMAF antibodies in pancreatic cancer cell lines. Capan-1 cells were treated for 12 hours at 37°C with 2.5 nM of 5F1-MMAF or 16B10-MMAF, with or without prior incubation with 50 µM of chloroquine (Ch), 3 hours before. White dots indicate colocalization between the anti-TGFα ADCs and LAMP1, which is represented by scatter graphs at the bottom. Scale: 25 µm. Anti-TGFα ADCs: red, LAMP1: green; DAPI: blue.