Synthesis, antifungal and antibacterial evaluation of trifluoromethylpyrimidine derivatives containing 1,3,4-thiadiazole and 1,2,4-triazole thioether moieties

In this study, twentythree trifluoromethylpyrimidine derivatives containing 1,3,4-thiadiazole and 1,2,4-triazole thioether moieties were designed and synthesized. These compounds were confirmed structurally through IR,¹H NMR,¹³C NMR, and HRMS analyses. Bioassay results revealed that some of the synthesized compounds exhibited higher antifungal activity compared to commercial drug. Particularly, compounds <b>7s</b> and <b>7t</b> demonstrated antifungal rates of 80.13% and 81.70% against <i>Sclerotinia sclerotiorum</i> at 50 <i>μ</i>g/mL, outperforming the control agent Pyrimethanil (68.39%). Furthermore, their EC₅₀ values of 14.68 and 13.06 <i>μ</i>g/mL, respectively, were better than that of Pyrimethanil (46.94 <i>μ</i>g/mL). These findings suggest that compounds <b>7s</b> and <b>7t</b> hold promise for future drug development due to their efficient synthesis and notable biological activities.