Additional file 2 of The RBM39 degrader indisulam inhibits acute megakaryoblastic leukemia by altering the alternative splicing of ZMYND8

Supplementary Material 2: Fig. S2. High epression of RBM39 is associated with poor prognosis and reduced RBM39 expression by shRNA inhibited AMKL cell survival. RBM39 expression in normal tissue and tumor samples was explored via the TCGA and GTEx databases. The TCGA and GTEx databases revealed that RBM39 was upregulated in AML samples compared with normal samples. TPM, transcripts per million. T, tumor/cancer. N, normal. Kaplan–Meier survival analysis of AML patients with high or low RBM39 expression using the TCGA and GTEx databases. The difference in prognosis was significant according to the log-rank test. RT-qPCR analysis of RBM39 mRNA expression in different AML cell lines. RT-qPCR was used to detect the knockdown efficiency of RBM39 in AMKL cell lines. Flow cytometry analysis of Annexin V + cells in the shNC and shRBM39 groups. Flow cytometry analysis of apoptotic cells in the shNC and shRBM39 groups. The error bars denote the SD. P values were determined via Mann–Whitney U test and are indicated as *P < 0.05, ***P < 0.001, and ****P < 0.0001. "ns" signifies not significant. "N" refers to biological replicates. Each experiment was performed with three technical replicates.