Additional file 4 of Linear ubiquitination of p31comet by HOIP couples cytokine response with mitotic regulation

Additional file 4: Figure S4. Delayed mitotic exit is observed in HOIP-depleted cells. A. Time-lapse microscopy of sgGFP and sgHOIP HeLa cells stably expressing a H2B-YFP retroviral construct. Representative images with higher resolution from the time point 60 min for sgGFP cells and the 120 min for sgHOIP cells shown in Figure 4F. B. IB analysis of WCL derived from HCT116 cells transduced with sgGFP (as the negative control) or sgHOIP lentiviral constructs. C-E. FACS analysis of sgGFP-HeLa (C) and sgHOIP-HeLa (D), which were fixed and stained with propidium iodide before being sorted using a BD FACSCanto II flow cytometer. Cell cycle phases were analyzed using FlowJo and compared in (E).