Additional file 1 of Targeting the hydrophobic pockets of FAK/PYK2 FAT domain: a highly effective inhibitory strategy suppressing tumor growth and eliminating metastasis

Additional file 1: Fig. S1. LD2-LD4 effectively displaces PYK2 from FAs in FAK null cells. Fig. S2. LD2-LD4 effectively displaces both PYK2 and FAK from FAs in U-118 MG cells. Fig. S3. Doxycycline has a strong anti-proliferative effect on MDA-MB-231 cells. Fig. S4. Doxycycline does not inhibit orthotopic MDA231-LM2- 4175 tumor growth and metastatic spread. Fig. S5. LM2-GFP cells detected in the lungs and abdominal region of non-treated and doxycycline-treated mice. Fig. S6. LD2-LD4 displaces PYK2 from focal adhesions. Fig. S7. Displacement of FAK and PYK2 from FAs by LD2-LD4 expression does not affect overall FA composition. Fig. S8. LD2-LD4 inhibits FAK kinase-dependent functions and downstream signaling in LM2 cells. Fig. S9. LD2-LD4 inhibits cell migration of SUM149 and SUM159 cells. Fig. S10. Single LD motifs do not displace FAK from focal adhesions. Fig. S11. Linker(3-4)-LD4 peptide expression inhibits tumor cell migration and invasion.