Additional file 1 of In-feed provision of binding proteins sustains piglet gut health and mitigates ETEC-induced post-weaning diarrhea

Additional file 1. Supplementary material of In-feed provision of binding proteins sustains piglet gut health and mitigates ETEC-induced post-weaning diarrhea. Table S1. Starter diet feed composition (Exp. A). Table S2. Pellet feed composition (Exp. B). Table S3. Monoclonal antibodies used for immunolabelling in flow cytometry analysis. Table S4. The daily intake (mg) of each VHH constructs per pig in Exp. A and Exp. B. Table S5. Plasma acute phase proteins (CRP, Haptoglobin) concentration of pigs in the experimental groups (Exp. A). Table S6. Body weight, average daily gain, average daily feed intake, gain to feed ratio (G:F), and rectal temperature of pigs in the experimental groups (Exp. B). Table S7. Morphological parameters of the small intestine in pigs in the experimental groups on day 4 post-challenge (Exp. B). Fig. S1. Gating strategy for analysis of granulocytes, monocytes, and lymphocyte subpopulations (CD45+, CD21+) in blood samples. Fig. S2. Gating strategy for analysis of lymphocyte subpopulations (CD3+, αβ T cells, γδ T cells, CD4+, CD8+, CD4+CD8+ cells) in blood samples. Fig. S3. Fecal consistency scores in Exp. A (based on a 7-point scale with scores 47 indicating diarrhea). Fig. S4. Fecal levels of F4+ ETEC (log CFU/g), shedding of est-II (STb toxin), eltB (LT toxin) gene (log copies/g) in pigs and body weight across experimental groups (Exp. A). Fig. S5. Dry matter content in fecal and digesta samples, and levels of eltB (LT toxin), est-II (STb toxin) gene (log copies/g) in pigs across experimental groups (Exp. B). Fig. S6. Representative images of H&E-stained sections of the mid (SI50) and distal (SI90) small intestine in pigs across experimental groups (Exp. B). Fig. S7. Flow cytometry analysis of peripheral blood lymphocyte subsets in pigs across experimental groups (Exp. B). Fig. S8. Epithelium gene expression in piglets across experimental groups (Exp. B).