Additional file 1 of Accelerating cartilage regeneration with DNA-SF hydrogel sustained release system-based cartilage organoids

Additional file 1: Materials and methods. Table S1 DNA sequence information (the bold sequences are the sticky ends). Table S2 Antibodies for Western blotting, IF, and IHC. Table S3 Primer sequences of the genes for qRT-PCR analysis. Fig. S1 Synthesis and characterization of DSRGT. Fig. S2 Models 3D-printed using a DLP system based on DSRGT. Fig. S3 Effects of DSR, DSRG, DSRT, and DSRGT on BMSC biocompatibility and spreading. Fig. S4 TD-198946 activates the Notch3 signaling pathway in BMSCs. Fig. S5 Evaluation of COs. Fig. S6 Histological staining and IHC staining results of regenerated cartilage at 4 and 8 weeks post-treatment. Fig. S7 Evaluation of regenerated cartilage at 4 and 8 weeks post-treatment. Fig. S8 In vivo toxicity evaluation. Fig. S9 Cartilage organoids (COs) promote cartilage regeneration by upregulating the MAPK pathway