Additional file 1 of Candidate Phyla Radiation (CPR) bacteria from hyperalkaline ecosystems provide novel insight into their symbiotic lifestyle and ecological implications

Additional file 1 : Figure S1. In the genomic context of two THF-auxotrophs (MAGs bin14 and bin24), no folA (K00287) was detected close to the gene thyA (K00560), while both folA and thyA genes were detected in the CPR genomes (i.e., MAGs bin31, bin34, and bin36). The folA and thyA genes were highlighted in yellow. The genes annotated using the KEGG database with KO numbers were highlighted in grey, while those without annotations were shown in white. The detailed information about the genomic context of the scaffold harboring folA or thyA of CPR bacteria and their potential THF-auxotrophic hosts was provided in Table S10. Figure S2. Amino acid sequence alignment of DHFRs. The conserved folate and NADP+ binding sites were highlighted with red and blue background, respectively.