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<b>Smart ionic liquid-based magnetic microextraction for ultra-selective detection of norcotamine (NCOT): A biomarker-driven analytical approach for smoking exposure and environmental assessment</b><br>Mehdi Hosseini^*, Hossein Papi<i>Department of Chemistry, Faculty of Basic Sciences, Ayatollah Boroujerdi University, Boroujerd, Iran.</i><br>Corresponding author e-mails: [email protected]; [email protected]<br><br><b>Alternative text in figure captions</b><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><b>Scheme 1</b>: A step-by-step chemical synthesis process of the smart ionic liquid [Hpyr][Cl], showing molecular structures and reaction pathways.<b>Scheme 2</b>: A simplified diagram illustrating how the ionic liquid [Hpyr][Cl] extracts NCOT from a sample and enables its detection.<b>Figure 1</b>: Four different spectra used to confirm the chemical structure of the synthesized ionic liquid: (A) Hydrogen NMR, (B) Carbon NMR, (C) FTIR spectrum, and (D) Magnetic property analysis of the modified ionic liquid.<b>Figure 2</b>: (A) Absorption spectra showing how NCOT and the ionic liquid interact, (B) A graph illustrating the relationship between the concentration of NCOT and its absorbance, and (C) A visual representation of the molecular interaction between NCOT and the ionic liquid based on theoretical calculations.<b>Figure 3</b>: (A) A molecular interaction map of NCOT and the ionic liquid with a color-coded scale, (B) A graph depicting the strength of molecular interactions, (C) A chart showing how the acidity of the sample affects NCOT extraction, (D) A graph indicating how the amount of ionic liquid influences NCOT extraction, (E) A study on how the extraction time affects NCOT efficiency, and (F) A chart demonstrating the effect of the magnetizing agent on NCOT extraction.