Additional file 1 of Mechanism of non-coding RNA regulation of DNMT3A

Supplementary Material 1. This article contains the following supporting information:Fig. S1. Functional characterization of RNA-mediated inhibition of DNMT3A_CDWT activity and selectivity of Fos-1 ecRNA for human DNMTs. Fig. S2. Computational models predict Fos-1 ecRNA binds at the tetramer interface of DNMT3A. Fig. S3. Modulation of DNMT3A_CDWT enzymatic activity by Fos-1 ecRNA is dominant in the presence of DNMT3L. Fig. S4. Binding of Full-length DNMT3AWT to FAM-labeled H3K4me0 peptides. Fig. S5. Excess Fos-1 ecRNA inhibits Full-length DNMT3AWT activity with polynucleosomes as substrates. Fig. S6. Summary gel of proteins used in this study. Fig. S7. Predicted secondary structures of RNAs used in this study. Fig. S7. Interactions between DNMT3A R771 and DNMT3L at the tetramer interface. Table S1. Target sequences and dyes of probes used in Single Molecule RNA FISH.