Additional file 1 of Receptor deorphanization in starfish reveals the evolution of relaxin signaling as a regulator of reproduction

Additional file 1. Fig. S1. BLOSUM62 cluster map of relaxin/insulin/IGF-type peptide precursors showing that the A. rubens RGP1 and RGP2 (ArubRGP1, ArubRGP2) and the A. cf. solaris RGP1 and RGP2 (AsolRGP1, AsolRGP2) precursors are positioned in a cluster that contains vertebrate relaxin precursors. Nodes are labelled with phylum-specific colours, shown in the key, and connections represent BLAST relationships with a P value > 1e-1. Precursors are labelled with different symbols in accordance with clades they are positioned in the phylogenetic tree shown in Fig. 1 and taxa colour coded (see key). The Asterias rubens and Acanthaster cf. solaris RGP1 and RGP2 precursors are labelled. Accession numbers for precursor sequences included in this figure are listed in Additional file 10: Dataset S3 and the sequences of the precursor proteins in FASTA format are listed in Additional file 11: Dataset S4.