Potential effects of Linh Loc Son hard capsule – a Vietnamese herbal combination in immunodeficiency induced by cyclophosphamide on mice

Context: Traditional medicine and herbal extracts have long been recognized for their immunomodulatory effects, enhancing patient immune responses. The use of traditional medicine to target specific pathogens and support the immune system is increasingly studied. Linh Loc Son (LLS) hard capsule, a product derived from nature, consists of the main four natural ingredients: Curculigo orchioides Gaertn., Dioscorea persimilis Prain et Burk., Morinda officinalis F.C.How. and Fallopia multiflora (Thunb.) Haraldson. Aims: To evaluate the potential effects of LLS hard capsules in complementary treatment of immunodeficiency state in mice. Methods: An experiment was conducted to assess the impact of LLS hard capsules on an immunosuppressed model of cyclophosphamide-induced Swiss mice of either sex. Two doses of LLS hard capsules (0.69 and 1.38 g/kg body weight) and levamisole (100 mg/kg body weight), serving as a positive control, were administered orally for seven consecutive days; cyclophosphamide (200 mg/kg i.p.) was administered on the fourth day. Results: Both doses of LLS hard capsule did insignificantly ameliorate the immunosuppressive effects of cyclophosphamide on the delayed-type hypersensitivity response but maintained leukocyte counts, relative organ weight, and cytokines as compared to the levamisole group and showed a significant improvement in micro-histological images. Conclusions: This study has demonstrated in vivo the immunostimulatory effect of LLS hard capsules, highlighting their potential to boost and regulate the immune response in mice models of immunodeficiency.