Molecular Characterization of Maize (Zea Mays L.) Genotypes Using Rapd Markers

Abstract The present investigation was undertaken to analyze the genetic diversity in 7 Maize genotypes viz. BTM -15, BTM – 02, BTM -04, BTM – 13, BTM – 11, Phule RAJASHRI, Phule SHAKTI for using RAPD analysis. RAPD amplified polymorphic DNA (RAPD) profile for were generated with 7 primers. Such as OPBF – 01, OPBF – 02, OPBF – 03, OPBF – 04, OPBF – 05, OPBF – 06, OPBF – 07. Seven primers generated 53 amplicons, of which 43 were polymorphic with an average of 5.3 amplicons per primer. A dendrogram was constructed by using the UPGMA that was based on similarity coefficients. The Jacquard’s similarity coefficient ranged from 0.46 to 0.82. Among the 7 Maize genotypes, Maximum similarity coefficient 0.82 was observed between BTM-04 and BTM-11 and least similarity coefficient 0.46 was observed between BTM-04 and Phule Shakti. Seven Maize genotype were clustered in two distinct groups that is cluster A and cluster B. Based on the dendrogram, the genotypes can be divided into two major clusters (A and B). Cluster A further splits into sub-clusters A1 and A2, and sub-cluster A2 divides again into two smaller sub-clusters: A2-1 and A2-2. This hierarchical grouping indicates the genetic diversity and relatedness among these Genotypes.