Torulaspora nezacadeziae M. Silva, F. Paraiso & J. P. Sampaio 2025

Torulaspora nezacadeziae M. Silva, F. Paraíso & J.P. Sampaio, sp. nov. MB 853982. Fig. 4U–W. Etymology: Torulaspora nezacadeziae. ne.za.ca.de’zi.ae, N.L. gen n. nezacadeziae, of Neža Čadež, in honour of Neža Čadež, in recognition of her contributions to yeast taxonomy. Typus: Japan, Chiba Prefecture, soil underneath Castanopsis sieboldii, 2008, Y. Imanishi (holotype PYCC 8102H, ex-holotype cultures PYCC 8102, CBS 18513). The holotype is permanently maintained in a metabolically inactive state in the Portuguese Yeast Culture Collection, Caparica, Portugal. The genome of this species was deposited at DDBJ /ENA/ GenBank under the accession GCA_964263675. The version described in this paper is v. 1. Description: After 1 wk on YM agar at 25 °C, cultures are smooth, cream-coloured, and butyrous. After 3 d of growth on YM agar at 25 °C, cells are globose (3–5 µm) to sub-globose (4–5 × 2–4 µm) and occur singly or in pairs, and proliferation is by multilateral budding (Fig. 4U). On Dalmau plates after 2 wk at 25 °C, no pseudohyphae nor true hyphae are formed. Sexual reproduction is observed on acetate agar after 15 d at 20 °C, and the cultures appear to be homothallic. Cellular extensions that resemble conjugation tubes are frequent and usually are not involved in conjugation. Asci are persistent and normally are formed after conjugation involving a cell and its bud. Asci produce one to three, possibly four, smooth and spherical ascospores, measuring 1.5–2.5 µm diam. (Fig. 4V, W). The physiological and biochemical profile of the species in shown in Table 1 and Table S5. Habitat and distribution: This species was found only once in soil underneath Castanopsis sieboldii, Chiba Prefecture, Japan.