UV Spectrophotometric Method Development and Validation for the Determination of Fluoxetine Hydrochloride in Bulk Drug and Commercial Formulation

The simple and rapid spectrophotometric technique has been developed to measure and validate the absorbance of antidepressant drug fluoxetine hydrochloride. This study's objective is to use UV spectroscopy to precisely and accurately estimate the maximum wavelength of the bulk drug and the pH at which the bulk drug exhibits the highest absorbance. According to Beer’s law concentration range of bulk drug and sample is 2-20µg/ml in buffer at pH 1.2, 6.8 and 7.4 respectively. The ℷmax of the bulk drug is 226nm. The linear regression equations of bulk drug were determined to be y = 00.0463x + 00.0602, y = 00.0417x + 00.0749, y = 00.0408x + 00. 0015 and for the marketed formulation found to be y = 00.0412x + 00.0825, y = 00.0386x + 00.0829, y = 00.0374x + 00.0816 in 1.2, 6.8 and 7.4 pH buffer respectively. The regression coefficient of each and every equation were found to be above 00.995 which denoted that all equations were following linearity.