Formulation and Evaluation of Wound Healing Gel Using Datura and Garlic Oil

This study focuses on the formulation and evaluation of a topical wound healing gel using natural extracts from Datura stramonium and garlic (Allium sativum) oil, both of which are known for their extensive therapeutic properties. The objective is to harness the analgesic, anti-inflammatory, and antimicrobial effects of Datura alongside the antibacterial, antifungal, and tissue-regenerating properties of garlic oil to develop a natural and effective wound treatment. The growing concern over the side effects and resistance associated with synthetic wound care products has driven interest in plant-based alternatives. The gel was formulated using Carbopol as a gelling agent, Aloe vera as a soothing base, and triethanolamine for pH adjustment. The Datura extract was obtained through ethanol maceration, ensuring the safe extraction of active alkaloids like atropine and scopolamine, while garlic oil was either purchased or lab-prepared using solvent extraction techniques. The gel underwent thorough physicochemical evaluation including pH (5.5), viscosity (35000cps), Spreadability (30g.cm/sec), and stability testing at different temperatures over three months, all of which confirmed its suitability for topical use. Antibacterial activity was confirmed against common wound pathogens (Staphylococcus aureus and Escherichia coli) using the agar well diffusion method, showing inhibition zones of 14 mm and 12 mm respectively. In vivo testing in albino rats with excisional wounds showed significantly faster wound closure in the treated group (90% by day 14) compared to the control (60%). Histopathological analysis further confirmed enhanced collagen deposition and tissue regeneration. These findings support the use of a Datura- and garlic oil-based gel as a natural, effective, and safe alternative to synthetic wound healing treatments. The formulation offers promising